Moving to Human Immunodeficiency Virus Type 1 Vaccine Efficacy Trials: Defining T Cell Responses As Potential Correlates of Immunity
Identifieur interne : 003242 ( Main/Exploration ); précédent : 003241; suivant : 003243Moving to Human Immunodeficiency Virus Type 1 Vaccine Efficacy Trials: Defining T Cell Responses As Potential Correlates of Immunity
Auteurs : Nina D. Russell [États-Unis] ; Michael G. Hudgens ; Richard Ha ; Colin Havenar-Daughton ; M. Juliana Mcelrath [États-Unis]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2003.
Abstract
There is evidence in both simian immunodeficiency virus and human immunodeficiency virus (HIV) type 1 infection that class I major histocompatibility complex–restricted CD8+ cytotoxic T lymphocytes play a pivotal role in controlling infection and, potentially, in protecting by immunization. Progress has been made in designing strategies to elicit these responses with HIV-1 vaccines, but methods to reproducibly quantify them have posed difficulties. An interferon-γ enzyme-linked immunospot assay, using peptide pools spanning the HIV-1 genes, was developed and standardized. This method is rapid (2 days), sensitive (threshold of detection, ⩾0.005%), quantitative, feasible using cryopreserved cells, and able to define epitope specificities. When this assay was applied to 36 HIV-1–seropositive and 10 HIV-1–seronegative subjects, it proved to be robust (specificity, 100%). When responses in natural infection were compared with vaccine-induced responses, vaccine recipient responses were ⩾1 log lower, which confirms the importance of using this sensitive assay as an initial screen in vaccine protocols
Url:
DOI: 10.1086/367702
Affiliations:
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Progress has been made in designing strategies to elicit these responses with HIV-1 vaccines, but methods to reproducibly quantify them have posed difficulties. An interferon-γ enzyme-linked immunospot assay, using peptide pools spanning the HIV-1 genes, was developed and standardized. This method is rapid (2 days), sensitive (threshold of detection, ⩾0.005%), quantitative, feasible using cryopreserved cells, and able to define epitope specificities. When this assay was applied to 36 HIV-1–seropositive and 10 HIV-1–seronegative subjects, it proved to be robust (specificity, 100%). When responses in natural infection were compared with vaccine-induced responses, vaccine recipient responses were ⩾1 log lower, which confirms the importance of using this sensitive assay as an initial screen in vaccine protocols</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><noCountry><name sortKey="Ha, Richard" sort="Ha, Richard" uniqKey="Ha R" first="Richard" last="Ha">Richard Ha</name><name sortKey="Havenar Daughton, Colin" sort="Havenar Daughton, Colin" uniqKey="Havenar Daughton C" first="Colin" last="Havenar-Daughton">Colin Havenar-Daughton</name><name sortKey="Hudgens, Michael G" sort="Hudgens, Michael G" uniqKey="Hudgens M" first="Michael G." last="Hudgens">Michael G. 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